CeleScope Report

Sample

Sample ID test1
Assay Single-cell rna
Chemistry scopeV2.0.1 (kit V1)
Software Version 1.12.0b0

Demultiplexing

Raw Reads 100,000
Valid Reads 74,870(74.87%)
Q30 of Barcodes 95.49%
Q30 of UMIs 88.11%

Trimming

Reads with Adapters 19,804(26.45%)
Reads Too Short 2,005(2.68%)
Reads Written 72,865(97.32%)
Base Pairs Processed 11,230,500
Base Pairs Quality-Trimmed 966,605(8.61%)
Base Pairs Written 9,041,813(80.51%)

Mapping

Genome Homo_sapiens_ensembl_99
Uniquely Mapped Reads 28,690(39.37%)
Multi-Mapped Reads 13,816(18.96%)

FeatureCounts

Feature Type Gene
Reads Assigned To Exonic Regions 21,509(74.97%)
Reads Assigned To Intronic Regions 1,460(5.09%)
Reads Assigned To Intergenic Regions 4,309(15.02%)
Reads Unassigned Ambiguity 1,412(4.92%)

Cells

Estimated Number of Cells 178
Fraction Reads in Cells 61.45%
Mean Reads per Cell 420
Median UMI per Cell 33
Total Genes 3,300
Median Genes per Cell 30
Saturation 15.88%

Analysis


Marker Genes by Cluster

cluster gene avg_log2FC pct.1 pct.2 p_val_adj